Extracellular vesicles (EVs) are membrane-derived particles surrounded by a phospholipid bilayer that are released by cells. We use PKH67 dye to efficiently label EV membranes. Then the excess unbound dyes are fast removed through our excellent Spin Columns from labeled exosome preparations, which separate molecules on the basis of the differences in size. Contaminant removal is easier and faster than traditional clean-up methods such as ultracentrifugation, spin filters.

With a highly specific membrane labeling and very low background levels, the kit can be used for most applications that require visualization of labeled EVs for tracking studies.